Business incubators provide the structural support that enables new businesses to grow and become financially independent, which in turn strengthens the private sector and propels an economy. In the last decade, Sub-Saharan Africa has seen a proliferation of business incubators, and it is hoped that they will play a key role in attaining the United Nations Sustainable Development Goals (SDGs), especially with regards to gender equality and the promotion of sustained and inclusive economic growth. Yet the effectiveness and impact of these business incubators remain poorly understood. Its role in accelerating the economic transformation of Africa is not well established. It is therefore important to evaluate the impact of these incubators in the African countries in which they operate, especially relative to specific SDGs. It is also essential to understand if these incubators comply with gender requirements, the socio-economic inclusion of women and youth, and how development finance institutions can strengthen the ecosystem.